Organelle proteomics.
نویسندگان
چکیده
This unit describes strategies for studying the proteomes of organelles, which is one example of targeted proteomics. It relies heavily on previously published units dealing with organelle preparation, protein solubilization, and proteomics techniques. A specific commentary for organelle proteomics is provided. Specific protocols for the isolation of nuclei from various sources (cell cultures, tissues) are also provided.
منابع مشابه
Proteomics of Saccharomyces cerevisiae Organelles*□S
Knowledge of the subcellular localization of proteins is indispensable to understand their physiological roles. In the past decade, 18 studies have been performed to analyze the protein content of isolated organelles from Saccharomyces cerevisiae. Here, we integrate the data sets and compare them with other large scale studies on protein localization and abundance. We evaluate the completeness ...
متن کاملProteomics of Saccharomyces cerevisiae Organelles.
Knowledge of the subcellular localization of proteins is indispensable to understand their physiological roles. In the past decade, 18 studies have been performed to analyze the protein content of isolated organelles from Saccharomyces cerevisiae. Here, we integrate the data sets and compare them with other large scale studies on protein localization and abundance. We evaluate the completeness ...
متن کاملOrganelle proteomics experimental designs and analysis.
In biology, localisation is function: knowledge of the localisation of proteins is of paramount importance to assess and study their function. This supports the need for reliable protein sub-cellular localisation assignment. Concomitant with recent technological advances in organelle proteomics, there is a requirement for more rigorous experimental and analysis design planning and description. ...
متن کاملIsolation of Vacuoles from Saccharomyces Cerevisiae
Any proteomics analysis starts with sample preparation. For organelle proteomics, where the aim is the identification of organelle-specific proteins, the sample preparation involves the isolation of an organelle of interest to a high degree of purity devoid of cross-contaminations from other organelles. Different organelles of the yeast Saccharomyces cerevisiae possess unique physical/chemical ...
متن کاملImproved sub-cellular resolution via simultaneous analysis of organelle proteomics data across varied experimental conditions.
Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among partially separated fractions. Such methods permit simultaneous analysis of un...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Current protocols in protein science
دوره Chapter 24 شماره
صفحات -
تاریخ انتشار 2006